Detection by real-time PCR and conventional culture of Salmonella Typhimurium and Listeria monocytogenes adhered to stainless steel surfaces under dry conditions

This study evaluated the capacity of real-time PCR and conventional culture methods to detect Salmonella enterica serovar Typhimurium Listeria monocytogenes adhered stainless steel surfaces used as food contact surfaces. The adhesion microorganisms was performed under dry conditions represent stress which these pathogens can be subjected in processing environment. samples were analyzed with various pre-enrichment times: S. Typhymurium (0, 6, 18 h) L. 25 procedures concentrating or not after pre-enrichments. results showed that obtained increased than method a low number both pathogens, even detected without pre-enrichment. However, is recommended avoid detection false positives from dead cells during ensure absence negatives due initial concentrations. concentration adhering bacteria frequency positive for Typhimurium, but this effect observed case . • Detection conditions. High compared method. Pre-enrichment positives. Concentration increases detection.